Major Publications

Platelet Metabolic Profiling Reveals Glycolytic and 1-Carbon Metabolites Are Essential for GP VI–Stimulated Human Platelets. Madankumar Ghatge, Gagan D. Flora, Manasa K. Nayak, Anil K.  Chauhan. Arterioscler Thromb Vasc Biol. 2023 November.  https://doi.org/10.1161/ATVBAHA.123.319821 (Online ahead of print)

A multi-laboratory preclinical trial in rodents to assess treatment candidates for acute ischemic stroke. Lyden PD, Diniz, MA Bosetti F, Lamb J, Nagarkatti KA, Rogatko A, Kim S, Cabeen RP, Koenig JI, Akhter K, Arbab AS, Avery BD, Beatty HE, Bibic A, Cao S, Boisserand LSB, Chamorro A, Chauhan A, Diaz-Perez S, Dhandapani K, Dhanesha N, Goh A, Herman AL, Hyder F, Imai T,  Johnson CW, Khan MB, Kamat P, Karuppagounder SS, Kumskova M, Mihailovic JM, Mandeville JB, Morais A, Patel RB, Sanganahalli BG, Smith C, Shi Y, Sutariya B, Thedens D, Qin T, Velazquez SE, Aronowski J, Ayata C, Chauhan AK, Leira EC, Hess DC, Koehler RC, McCullough LD, Sansing LH. Science Translational Medicine. 2023 Sep 20;15(714):eadg8656 doi:10.1126/scitranslmed.adg8656 (Online ahead of print).

Targeting Neutrophil α9 Improves Functional Outcomes After Stroke in Mice With Obesity-Induced Hyperglycemia. Patel RB, Dhanesha N, Sutariya B, Ghatge M, Doddapattar P, Barbhuyan T, Kumskova M, Leira EC, Chauhan AK. Stroke. 2023 Jul 14. https://doi.org/10.1161/STROKEAHA.123.042714 (Online ahead of print).

Characterization of bleeding symptoms in Ehlers–Danlos syndrome. Kumskova M, Flora GD, Staber J, Lentz SR, Chauhan AK. J. Thromb. Haemost. 2023 Jul 1;21(7):1824-30. doi:https://doi.org/10.1016/j.jtha.2023.04.004

Mitochondrial calcium uniporter b deletion inhibits platelet function and reduces susceptibility to arterial thrombosis. Ghatge M, Nayak MK, Flora GD, Kumskova M, Jain A, Patel RB, Lin Z, Usachev YM, Chauhan AK.  J. Thromb. Haemost. 2023 Aug 1;21(8):2163-2174. https://doi.org/10.1016/j.jtha.2023.04.002. 

Mitochondrial pyruvate dehydrogenase kinases contribute to platelet function and thrombosis in mice by regulating aerobic glycolysis. Flora GD, Nayak MK, Ghatge M, Kumskova M, Patel RB, Chauhan AK.  Blood Adv 2023 Jun 13;7(11):2347-59. https://doi.org/10.1182/bloodadvances.2023010100. 

Myeloid Cell PKM2 Deletion Enhances Efferocytosis and Reduces Atherosclerosis. Doddapattar P, Dev R, Ghatge M, Patel RB, Jain M, Dhanesha N, Lentz SR, Chauhan AK. Circ Res. 2022 Apr 11:101161CIRCRESAHA121320704. doi: 10.1161/CIRCRESAHA.121.320704. Epub ahead of print. 

The Stroke Preclinical Assessment Network: Rationale, Design, Feasibility, and Stage 1 Results. Lyden PD, Bosetti F, Diniz MA, Rogatko A, Koenig JI, Lamb J, Nagarkatti KA, Cabeen RP, Hess DC, Kamat PK, Khan MB, Wood K, Dhandapani K, Arbab AS, Leira EC, Chauhan AK, Dhanesha N, Patel RB, Kumskova M, Thedens D, Morais A, Imai T, Qin T, Ayata C, Boisserand LSB, Herman AL, Beatty HE, Velazquez SE, Diaz-Perez S, Sanganahalli BG, Mihailovic JM, Hyder F, Sansing LH, Koehler RC, Lannon S, Shi Y, Karuppagounder SS, Bibic A, Akhter K, Aronowski J, McCullough LD, Chauhan A, Goh A; SPAN Investigators. Stroke. 2022 Mar 31:101161STROKEAHA121038047. doi: 10.1161/STROKEAHA.121.038047. Online ahead of print.

PKM2 promotes neutrophil activation and cerebral thromboinflammation: therapeutic implications for ischemic stroke.  Dhanesha N, Patel RB, Doddapattar P, Ghatge M, Flora GD, Jain M, Thedens D, Olalde H, Kumskova M, Leira EC, Chauhan AK. Blood. 2022 Feb 24;139(8):1234-1245.  

The metabolic enzyme pyruvate kinase M2 regulates platelet function and arterial thrombosis. Nayak MK, Ghatge M, Flora GD, Dhanesha N, Jain M, Markan KR, Potthoff MJ, Lentz SR, Chauhan AK. Blood. 2020 Oct 7:blood.2020007140

Differential Roles of Endothelial Cell-Derived and Smooth Muscle Cell-Derived Fibronectin Containing Extra Domain A in Early and Late Atherosclerosis. Doddapattar P, Dev R, Jain M, Dhanesha N, Chauhan AK. Arterioscler Thromb Vasc Biol. 2020 Jul;40(7):1738-1747

Targeting Myeloid-Specific Integrin α9β1 Improves Short- and Long-Term Stroke Outcomes in Murine Models With Preexisting Comorbidities by Limiting Thrombosis and Inflammation. Dhanesha N, Jain M, Tripathi AK, Doddapattar P, Chorawala M, Bathla G, Nayak MK, Ghatge M, Lentz SR, Kon S, Chauhan AK. Circ Res. 2020 Jun 5;126(12):1779-1794.

Targeting Myeloid-Cell Specific Integrin α9β1 Inhibits Arterial Thrombosis in Mice. Dhanesha N, Nayak MK, Doddapattar P, Jain M, Flora GD, Kon S, Chauhan AK. Blood. 2020 Jan 17. pii: blood.2019002846

Smooth muscle cell-specific fibronectin-EDA mediates phenotypic switching and neointimal hyperplasia. Jain M, Dhanesha N, Doddapattar P, Chorawala MR, Nayak MK, Cornelissen A, Guo L, Finn AV, Lentz SR, Chauhan AK. J Clin Invest. 2020 Jan 2;130(1):295-314

Fn-EDA (Fibronectin Containing Extra Domain A) in the Plasma, but Not Endothelial Cells, Exacerbates Stroke Outcome by Promoting Thrombo-Inflammation. Dhanesha N, Chorawala MR, Jain M, Bhalla A, Thedens D, Nayak M, Doddapattar P, Chauhan AK. Stroke. 2019 May;50(5):1201-1209

Dichloroacetate, an inhibitor of pyruvate dehydrogenase kinases, inhibits platelet aggregation and arterial thrombosis. Nayak MK, Dhanesha N, Doddapattar P, Rodriguez O, Sonkar VK, Dayal S, Chauhan AK. Blood Adv. 2018 Aug 14;2(15):2029-2038. 

Endothelial Cell-Derived Von Willebrand Factor, But Not Platelet-Derived, Promotes Atherosclerosis in Apolipoprotein E-Deficient Mice. Doddapattar P, Dhanesha N, Chorawala MR, Tinsman C, Jain M, Nayak MK, Staber JM, Chauhan AK. Arterioscler Thromb Vasc Biol. 2018    

ADAMTS13 Retards Progression of Diabetic Nephropathy by Inhibiting Intrarenal Thrombosis in Mice. Dhanesha N, Doddapattar P, Chorawala MR, Nayak MK, Kokame K, Staber JM, Lentz SR, Chauhan AK. Arterioscler Thromb Vasc Biol. 2017 Jul;37(7):1332-1338.

Genetic Ablation of Extra Domain A of Fibronectin in Hypercholesterolemic Mice Improves Stroke Outcome by Reducing Thrombo-Inflammation. Dhanesha N, Ahmad A, Prakash P, Doddapattar P, Lentz SR, Chauhan AK. Circulation. 2015 Dec 8;132(23):2237-47

Fibronectin Splicing Variants Containing Extra Domain A Promote Atherosclerosis in Mice Through Toll-Like Receptor 4. Doddapattar P, Gandhi C, Prakash P, Dhanesha N, Grumbach IM, Dailey ME, Lentz SR, Chauhan AK. Arterioscler Thromb Vasc Biol. 2015 Nov;35(11):2391-400.

Cellular fibronectin containing extra domain A promotes arterial thrombosis in mice through platelet Toll-like receptor 4. Prakash P, Kulkarni PP, Lentz SR, Chauhan AK. Blood. 2015 May 14;125(20):3164-72